| 质粒名称: | pC0078 RESCUE |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 130661 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 载体出品公司: | pcDNA3.1 |
| 空载体大小: | 9861 bp |
| 完整质粒大小: | 9864bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | dRanCas13b-ADAR2dd (RESCUE) |
| 物种来源: | H. sapiens (human) |
|
文献引用方法 |
|
| 材料和方法部分: | pC0078 RESCUE was a gift from Feng Zhang (Addgene plasmid # 130661 ; http://n2t.net/addgene:130661 ; RRID:Addgene_130661) |
| 参考文献部分: | A cytosine deaminase for programmable single-base RNA editing. Abudayyeh OO, Gootenberg JS, Franklin B, Koob J, Kellner MJ, Ladha A, Joung J, Kirchgatterer P, Cox DBT, Zhang F. Science. 2019 Jul 11. pii: science.aax7063. doi: 10.1126/science.aax7063. 10.1126/science.aax7063 PubMed 31296651 |
买家导航
pC0078 RESCUE载体质粒基本信息
pC0078 RESCUE质粒图谱载体图谱和pC0078 RESCUE载体序列质粒序列多克隆位点信息
pC0078 RESCUE质粒载体简介
pC0078 RESCUE质粒序列载体序列
LOCUS pC0078 RESCUE 9864 bp DNA circular SYN 25-AUG-2024
DEFINITION pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/
N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9864)
AUTHORS Abudayyeh OO, Gootenberg JS, Franklin B, Koob J, Kellner MJ, Ladha
A, Joung J, Kirchgatterer P, Cox DBT, Zhang F
TITLE A cytosine deaminase for programmable single-base RNA editing.
JOURNAL Science. 2019 Jul 11. pii: science.aax7063. doi:
10.1126/science.aax7063.
PUBMED 31296651
REFERENCE 2 (bases 1 to 9864)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Science.
2019 Jul 11. pii: science.aax7063. doi: 10.1126/science.aax7063."
FEATURES Location/Qualifiers
source 1..9864
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 381..584
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 629..648
/label=T7
/note="T7 promoter, forward primer"
promoter 629..647
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 683..692
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
primer_bind complement(5221..5238)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5227..5451
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5497..5925
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5584..5603)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5794..5815
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5934..5954)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5939..6268
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6119..6254
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6181..6200
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 6335..7129
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6389..6408)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 6999..7018
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 7303..7424
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7340..7359)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7394..7413
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7473..7489)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7473..7489)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7486..7508)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7497..7513
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7521..7551)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7566..7587
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7704..7721)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7875..8463)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7955..7974)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8634..9494)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9257..9276
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9495..9599)
/gene="bla"
/label=AmpR promoter
primer_bind complement(9674..9693)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
661 gctagcgttt aaacttaagc ttgccaccat ggagaagccc ctgctgccta acgtgtatac
721 cctgaagcac aagttctttt ggggcgcctt cctgaatatc gcccggcaca acgcctttat
781 caccatctgc cacatcaatg agcagctggg cctgaaaacc cccagcaacg acgataagat
841 cgtggacgtg gtgtgcgaga catggaacaa tatcctgaac aatgaccacg atctgctgaa
901 gaagtcccag ctgaccgagc tgatcctgaa gcacttccct tttctgacag ccatgtgcta
961 ccacccccct aagaaggagg gcaagaagaa gggccaccag aaggagcagc agaaggagaa
1021 ggagagcgag gcacagtccc aggcagaggc cctgaaccca tccaagctga tcgaggccct
1081 ggagatcctg gtgaatcagc tgcactctct ggcgaactac tatagcgcct ataagcacaa
1141 gaagcccgac gccgagaagg atatcttcaa gcacctgtat aaggccttcg acgcctctct
1201 gagaatggtg aaggaggatt ataaggccca cttcaccgtg aatctgacaa gggactttgc
1261 ccacctgaac cgcaagggca agaacaagca ggacaatccc gacttcaaca gatacagatt
1321 cgagaaggat ggcttcttta ccgagtctgg cctgctgttc tttacaaatc tgtttctgga
1381 caagagggat gcctattgga tgctgaagaa ggtgtccggc ttcaaggcct ctcacaagca
1441 gcgcgagaag atgaccacag aggtgttttg caggagccgc atcctgctgc ccaagctgag
1501 gctggagtcc cgctacgacc acaaccagat gctgctggat atgctgtctg agctgagcag
1561 atgtcctaag ctgctgtatg agaagctgag cgaggagaat aagaagcact tccaggtgga
1621 ggccgacggc tttctggatg agatcgagga ggagcagaac ccattcaagg acaccctgat
1681 ccggcaccag gatagattcc cctactttgc cctgaggtat ctggacctga atgagtcctt
1741 caagtctatc cgctttcagg tggatctggg cacataccac tattgtatct acgacaagaa
1801 gatcggcgat gagcaggaga agaggcacct gacccgcaca ctgctgtcct tcggccggct
1861 gcaggacttt accgagatca acagacccca ggagtggaag gccctgacca aggacctgga
1921 ttacaaggag acatccaatc agcctttcat ctctaagacc acaccacact atcacatcac
1981 cgacaacaag atcggctttc ggctgggcac aagcaaggag ctgtacccct ccctggagat
2041 caaggatggc gccaatagaa tcgccaagta cccttataac tccggcttcg tggcccacgc
2101 ctttatctct gtgcacgagc tgctgcctct gatgttctac cagcacctga ccggcaagag
2161 cgaggacctg ctgaaggaga cagtgcggca catccagaga atctataagg acttcgagga
2221 ggagcggatc aataccatcg aggatctgga gaaggcaaac cagggcagac tgccactggg
2281 agcctttccc aagcagatgc tgggcctgct gcagaataag cagcctgatc tgtccgagaa
2341 ggccaagatc aagatcgaga agctgatcgc cgagacaaag ctgctgtctc acaggctgaa
2401 cacaaagctg aagagctccc caaagctggg caagcggaga gagaagctga tcaagacagg
2461 cgtgctggcc gactggctgg tgaaggattt catgcgcttt cagcccgtgg cctacgacgc
2521 ccagaatcag cctatcaagt ctagcaaggc caactccacc gagttctggt ttatcaggcg
2581 cgccctggcc ctgtacggag gagagaagaa taggctggag ggctatttca agcagaccaa
2641 cctgatcggc aacacaaatc cacacccctt cctgaacaag tttaattgga aggcctgcag
2701 gaatctggtg gatttctacc agcagtatct ggagcagcgc gagaagtttc tggaggccat
2761 caagaaccag ccatgggagc cctaccagta ttgcctgctg ctgaagatcc ctaaggagaa
2821 caggaagaat ctggtgaagg gatgggagca gggaggaatc tctctgccac ggggcctgtt
2881 taccgaggcc atcagagaga cactgtctga ggacctgatg ctgagcaagc caatccgcaa
2941 ggagatcaag aagcacggcc gggtgggctt catcagcaga gccatcaccc tgtactttaa
3001 ggagaagtat caggataagc accagagctt ctacaatctg tcctataagc tggaggcaaa
3061 ggcaccactg ctgaagcggg aggagcacta cgagtattgg cagcagaaca agcctcagtc
3121 tccaaccgag agccagaggc tggagctgca cacaagcgac cgctggaagg attacctgct
3181 gtataagaga tggcagcacc tggagaagaa gctgcggctg taccggaatc aggacgtgat
3241 gctgtggctg atgaccctgg agctgacaaa gaaccacttc aaggagctga acctgaatta
3301 tcaccagctg aagctggaga atctggccgt gaacgtgcag gaggccgatg ccaagctgaa
3361 ccctctgaat cagaccctgc ccatggtgct gcctgtgaag gtgtaccctg ccacagcctt
3421 tggcgaggtg cagtaccaca agaccccaat caggacagtg tatatccgcg aggagcacac
3481 caaggccctg aagatgggca acttcaaggc cctggtgaag gaccggagac tgaatggcct
3541 gttctctttt atcaaggagg agaacgatac acagaagcac ccaatcagcc agctgcggct
3601 gaggcgcgag ctggagatct accagtctct gagagtggac gccttcaagg agacactgag
3661 cctggaggag aagctgctga ataagcacac atccctgtcc tctctggaga acgagtttag
3721 ggccctgctg gaggagtgga agaaggagta tgccgccagc tccatggtga cagacgagca
3781 catcgccttc atcgccagcg tggccaatgc cttctgcgcc aaccagtacc ccttctacaa
3841 ggaggccctg cacgccccta tcccactgtt caccgtggcc cagcccacca cagaggagaa
3901 ggatggcctg ggaatcgcag aggccctgct gaaggtgctg agggagtact gtgagatcgt
3961 gaagtcccag atcggatcca gcctccaaaa aaagttggag gaattagaac taggatccca
4021 gctgcattta ccgcaggttt tagctgacgc tgtctcacgc ctggtcatag gtaagtttgg
4081 tgacctgacc gacaacttct cctcccctca cgctcgcaga ataggtctgg ctggagtcgt
4141 catgacaaca ggcacagatg ttaaagatgc caaggtgata tgtgtttcta caggatctaa
4201 atgtattaat ggtgaatacc taagtgatcg tggccttgca ttaaatgact gccatgcaga
4261 aatagtatct cggagatcct tgctcagatt tctttataca caacttgagc tttacttaaa
4321 taacgaggat gatcaaaaaa gatccatctt tcagaaatca gagcgagggg ggtttaggct
4381 gaaggagaat atacagtttc atctgtacat cagcacctct ccctgtggag atgccagaat
4441 cttctcacca catgaggcaa tcctggaaga accagcagat agacacccaa atcgtaaagc
4501 aagaggacag ctacggacca aaatagaggc tggtcagggg acgattccag tgcgcaacaa
4561 tgcgagcatc caaacgtggg acggggtgct gcaaggggag cggctgctca ccatgtcctg
4621 cagtgacaag attgcacgct ggaacgtggt gggcatccag ggatcactgc tcagcatttt
4681 cgtggagccc atttacttct cgagcatcat cctgggcagc ctttaccacg gggaccacct
4741 ttccagggcc atgtaccagc ggatctccaa catagaggac ctgccacctc tctacaccct
4801 caacaagcct ttgctcacag gcatcagcaa tgcagaagca cggcagccag ggaaggcccc
4861 catattcagt gtcaactgga cggtaggcga ctccgctatt gaggtcatca acgccacgac
4921 tgggaaggga gagctgggcc gcgcgtcccg cctgtgtaag cacgcgttgt actgtcgctg
4981 gatgcgtgtg cacggcaagg ttccctccca cttactacgc tccaagatta ccaagcccaa
5041 cgtgtaccat gagacaaagc tggcggcaaa ggagtaccag gccgccaagg cgcgtctgtt
5101 cacagccttc atcaaggcgg ggctgggggc ctgggtggag aagcccaccg agcaggacca
5161 gttctcactc acgtaagcgg ccgctcgagt ctagagggcc catttaaacc cgctgatcag
5221 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
5281 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
5341 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
5401 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
5461 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
5521 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
5581 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
5641 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
5701 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
5761 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
5821 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
5881 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
5941 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
6001 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
6061 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
6121 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
6181 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
6241 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
6301 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
6361 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
6421 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
6481 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
6541 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
6601 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
6661 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
6721 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
6781 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
6841 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
6901 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
6961 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
7021 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
7081 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
7141 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
7201 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
7261 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
7321 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
7381 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
7441 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
7501 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
7561 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
7621 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
7681 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
7741 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
7801 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
7861 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
7921 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
7981 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
8041 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
8101 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
8161 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
8221 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
8281 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
8341 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
8401 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
8461 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
8521 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
8581 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
8641 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
8701 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
8761 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
8821 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
8881 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
8941 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
9001 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
9061 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
9121 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
9181 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
9241 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
9301 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
9361 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
9421 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
9481 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
9541 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
9601 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
9661 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
9721 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
9781 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
9841 atgtacgggc cagatatacg cgtt
//
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